Tag: DIYbio

Dazzling FireBall: Space Junk, UFO or AI Swarm distribution coverup

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While we were in residence at Scientific Delirium Madness a large fireball was seen in the sky over Palo Alto. Actually it was seen from San Francisco down to Los Angeles at the same time and it made it to Nevada and Utah before breaking up. The standard news took a few days to debate what the slow, glowing, giant comet-mass was. Delta Aquarid Perseid Meteor Shower ice ball? Flaming commercial airliner death ball? Off track or off map space junk?

Two days afterwards, Jonathan McDowell, astronomer of the Harvard Smithsonian Center for Astrophysics tweeted, “Observation reports from Utah indicate the second stage from the first Chang Zheng 7 rocket, launched Jun 25, reentered at 0440 UTC.” So, officially we have an identified flying object, synopsis… Chinese space junk. It was 36 feet long, 10 cubic meters, about the size of a school bus. Yes it was a big mofo. It scared lots of folx into thinking it was armageddon, a missile attack or an alien invasion. Capt. Nick Mercurio from Joint Functional Component Command for Space at Vandenburg Air Force Base, said “Given the size of the rocket body, some pieces of it may have survived re-entry but it’s unlikely it posed any threat to people.”

I can tell you that there was some curious followings along the trajectory of our CZ-7 Rocket booster flame out. The skies had f-19s scrambling the first day and copters with surface to air missiles the second day. They seemed to be tracking the path of the Chinese space manufacturing drizzle. Perhaps looking for covert pico-cricket robot swarms, Zika glossolaia attenuators, silicon eating 味噌 tauco 豆醬 fungal strains or rapid prototyping AI bunker camoCNC Router malevolent machine superintelligence (see http://leonardo.info/blogs/djerassi-field-notes-asa-calow/ ).

Be aware that being from Woodstock NY allows for conspiracy theory to be wide and far fetched. When presented with a school bus sized surprise space junk sky show, it is not the worst independent maneuver to free associate worst case scenarios… international subversions, government cover-ups, even wildhair, keef brained kook theory. In my humble opinion 60% of X-files style panic nutball theory turns out to be closer to the actual than baseline liar-liar mass media CNNFOX whitewash.

Track yourself as space junk:

https://www.space-track.org/

Uranus’ Castrated Penis: centiSperm Glazed Ceramic Sculpture

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centiSperm Glazed Ceramic tribute to Robert Arneson and the NorCal Funk Artists

centiSperm Glazed Ceramic tribute to Robert Arneson and the NorCal Funk Artists

The centiSperm was applied to Uranus’ Castrated Penis as a glaze. The sculpture fired well. The centisperm effect is basically un-perceptible. Yet, there is a pearly sheen to the penis of Uranus. Certainly, the ritual process of anointing the lingam, even the lingam forcibly removed, is of discerning taste. Here are some pictures of Uranus’ Castrated Penis. This sculpture is a tribute to Robert Arneson (a former teacher) and the NorCal Funk Artists.

tribute to Robert Arneson and the NorCal Funk Artists

tribute to Robert Arneson and the NorCal Funk Artists

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Flight of the centiSperm

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Flight of the centiSperm
David Bowen
a collaboration with Adam Zaretsky

So David Bowen came up with this idea of using the centiSperm as a data set for flying his drone. He and Asa Calow shit talked some tech and David built a rotating four well conductivity meter dubbed the Jizalizer 3030.

Jizilizer 3030 - David Bowen

Jizilizer 3030 – David Bowen

centiSPerm (Human sperm combined with DNA from a centipede) was used as a data set. Relative electrical flow was measured over time from the centiSperm producing data as the cells containing it decayed. This data was scaled and correlated to Latitude, Longitude and Altitude respectively. The XYZ/time data plot is neither devoid of the potentially legible nor is it easy to find meaning in. However, a fabulous graphic plotting of the loci was produced and it a find piece of the Jizalizer 3010 centiSperm continuum.

XYZ/time Jizalizer 3010 centiSperm data plot drone conversion path - David Bowen

XYZ/time Jizalizer 3010 centiSperm data plot drone conversion path – David Bowen

The coordinates were uploaded to the drone and it flew autonomously based on the DNA instructions. Weidong Yang took a fabulous time-lapse photograph of the flight at night. The LEDs left a trail of four-dimensional semidata in the sky.

autonomous drone path based on centisperm data instructions - David Bowen Timelaspe Photo Credit: Weidong Yang

autonomous drone path based on centisperm data instructions – David Bowen
Timelaspe Photo Credit: Weidong Yang

autonomous drone path based on centisperm data instructions - David Bowen Timelaspe Photo Credit: Weidong Yang

autonomous drone path based on centisperm data instructions – David Bowen
Timelaspe Photo Credit: Weidong Yang

autonomous drone path based on centisperm data instructions - David Bowen Timelaspe Photo Credit: Weidong Yang

autonomous drone path based on centisperm data instructions – David Bowen
Timelaspe Photo Credit: Weidong Yang

The use of a drone for data point plotting made for some complex readings. As you can see there are distinct coordinates of chaos on the stability locus of the flight path. The pauses and directional flavor of the Flight of the centiSperm led to group quizzical guesses as to what the 4D trail might imply.

Electroporation Protocol, the making of centiSperm

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Electroporation, the making of centiSperm

Comic Rendition:  Matteo Farinella

Matteo Farinella of http://matteofarinella.com

A – Mix sperm and DNA in the electroporation vial (cuvette)

For each reaction mix 10 microliters of centipede DNA and 50 microliters of desalinated sperm directly inside the electroporation cuvette

cuvette_centiSperm_4175

B – Put cuvette inside of the electroporator

C – Set voltage

I did one reaction at 200 volts which seemed standard in the sperm electroporation. I also ran a second reaction at 1800 volts just to make it rough. Sometimes when you are running a rough DIY lab its better to go for overkill than treading to closely to the protocol. More is sometimes a matter of affect. But more actually often does the trick!

D – Press button twice to fire machine. Wait for beep (about 2 seconds)
E – apply refresh media

For this lab I used 10% vitaminwater® XXX açai-blueberry- pomegranate flavored (Glaceau) put through a 3 micron Gelman Filter

 

 

 

 

 

centiSperm_4181

The refresh media was prepared before the electroporation reaction.

 

 

Untitled-48

photo credit: David Bowen

 

 

 

 

 

 

 

 

electroporator

     centiSperm as a new media is now available for experimentation.   

 

 

 

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centiSperm DIY Protocol: Sperm Desalination

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Goals: To electroporate whole genomic DNA from a centipede into the center of human sperm.

 

mateo-the-lab-centiSFIN

 

Matteo Farinella of http://matteofarinella.com

Matteo Farinella of http://matteofarinella.com

 

Sperm Desalination

Rinsing the sperm is meant to remove the salt from the sample. This is to reduce the electrocution risk to the sperm during electroporation. The electroporator gives high voltage millisecond pulses and if the sperm is in a salty seminal fluid medium, the electroporator will just zap the sperm to death. The process is much like being a human washing machine. The actual process is slow, boring and repetitive: spin, drain liquids, agitation rinse, spin, drain liquids, agitation rinse, spin, drain liquids, agitate into a sort of gel. Matteo was heard to say, “This is why I stopped being a scientist.”

A – Obtain Sample

Untitled-47

B – Put sperm in tubes

I had very few tubes with me. I thought to use disposable pipettes but I got worried about sperm spray because the tubes would have no tops. I bought hypodermic needles at the Wallgreens to use as sterile tubes. I figured that the syringes could be spun and the heavier sperm would deposit the pellet onto the black rubber plunger and then I could push out the liquid (supernatant) and suck in new liquid to resuspend into (vibrating agitation rinse). I used electrical tape to secure the needle closed. I used the screw-on.twist-off luer lock for as the cap for the syringe tube. This made accidental sharps sperm injection much less likely! I also taped the plunger closed so it wouldn’t fly off the fan releasing sperm in a circular spew pattern. I left tape slack so I could still plunge without having to worry about coating the place with a thin film of half rinsed ejaculate.

FANclose_centiSperm_4064

 

C – Spin to make pellet (natant)

I was going to buy a hand-powered centrifuge but I thought better of it. I tried to make due with what I had brought and immediate materials. Although a DIY handheld grinder centrifuge was beta tested. The results were dangerous. David Bowen and I put the grinder in a vice and stepped out of the shop to plug it in. The attachment took air, collided with the vice and self destructed sending shrapnel in every direction. We were nearly maimed in the face by a projectile syringe.

Beta Test Grinder Centrifuge Epic Fail

Beta Test Grinder Centrifuge Epic Fail

The DIY box fan centrifuge was the answer. I drilled holes in the plastic fan blades and zip tied the syringes to the blades of the fan. It took a little time to cut them off between each cycle but we did see a thin white line of sperm solids around the plunger after each spin.

FANcentiSperm_4061

 

Spin for about 3 minutes

D – Throw away supernatant (liquid)

Plunge the seminal fluid supernatant into the bleach bath.

A word on DIY Biosafety: When working with human fluids, it is good to have art as a shield from the health department. Art is not usually considered to be stringently regulated, as are federally funded laboratories and biobased economy manufacturing processing plants. Using somewhat dangerous materials in performance art or DIY labs is generally ignored. Not that the health department doesn’t have jurisdiction. There are just some funny ideas about art. It is generally considered to be sort of like a party in the ambi-bathroom of a good afterhours club. Art is a sleazy urinal cake. So, the mythos of general consensus art freedom from oversight is sort of a mix between fourth amendment freedom of expression issues and that soldier of fortune motto ‘kill em all, let god sort em out’. This is the attitude towards the arts and artist’s in general society. This is to say that the impact of art is zero to nil in the public eye and the desperate narcissism of most artists doesn’t hinder this vision. Proclaiming yourself an artist is like wearing a classless green translucent plastic masochist visor on your head. Artists signify as frivolous, expendable and hence deregulated. It is sort of an unsaid authoritative death wish hex that translates to freedom from funding and freedom to self regulate. Nonetheless, I used a water purification pill good for 25 liters in a bin as a bleach bath for both used fluids and plastic disposable trash. No harm in sterilizing the shit out of stuff, neh?

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Just suck distilled water into the syringe. Use the syringe plunger to fill the syringe with distilled water. Don’t forget to leur lock the sealed needle back on the syringe so there is minimal leakage.

F – Vortex

Resuspend sperm in distilled water by vortexing

A vortex is a great home toy to invest in. It is an industrial strength vibrating twiwrler. Its major use in a lab is for resuspending tacky solids stuck to the bottom of tubes after centrifugation. It can also be used a fun drink mixer. It does make that tell tale tornado in fluids pressed onto the nob of the vortex. The strength and RPM is well beyond the standard vibrator so there are other more potent uses for a home vortex related to: massage, deep tissues, and earthshaking stimulation possibilities. Well I left my home vortex at home. The DIY vortex for resuspension was simply to attach two syringe wrappers to a handheld sander and let the sander shake and vibrate the sperm solids back into solution with the saltless distilled water rinse. Sometimes I would press the hypodermics onto the sander surface to see the liquids really shaking and splashing and spinning and waving about. It worked! It was a little foamy, frothy for professional use, but the saucy was scenic.

G – Spin to make pellet (natant

Spin for about 5 minutes

H – Throw away supernatant (liquid)

Plunge the distilled water seminal rinse supernatant into the bleach bath

I – Replace distilled water with ice cold distilled water

J – Vortex
Just another jot on foam and happenstance.

Photo credit: David Bowen

Photo credit: David Bowen

The foam of castration, vortex and sperm, the birth of Aphrodite
Photo credit: David Bowen

There was some leakage of sperm from inside the hypodermics to outside on the surface. Good thing this lab was mostly stag. Gloves were offered and the dangers explained. But, during vortexing, the shaking and vibrating of the lost droplets of sperm appeared as foam. This again confirms that sperm, left out and agitated by waves, does make a foam! This foam is thick, meringue-like, white, bubbly, a shave cream consistency, a lather of leaky fluffed goo spread. Its an epic yuck factor emollient. It’s also proof of concept in the foam as castrato sperm meets ocean vortex theory of Aphrodite’s origin.

K – Spin to make pellet

L – Throw away supernatant

M – Replace ice cold distilled water with ice cold Glycerol

I bought glycerine at an uptown health and beauty herb pharmacopeia called Pharmaca in PaloAlto. It was the cheapest item in the storehouse of flower dew remedies and herbal tinctures. Glycerol is 10% glycerine. That is 9 parts distilled water and one part glycerine. A solution of glycerol is often used to keep just to keep mixtures suspended in a smooth, moisturizing solution. It is also a sort of preservative. For vegetarians, there is plant-based glycerol. Glycerol is used in lots of stuff we use every day. You can find it in shampoo, water based sex lubricants and in this case, a suspension of sperm. If well screened, young sperm suspension with a little fragrance, perhaps lavender, might make a very good face and cuticle cream for the boutique beauty product market.

SK-II Women's R.N.A.POWER Radical New Age Cream/2.7 oz. - Size No Size $230.00 Saks Fifth Avenue

SK-II Women’s R.N.A.POWER Radical New Age Cream/2.7 oz. – centiSperm humectance, one hundred handed face massage
$2300.00
Saks Fifth Avenue