Electroporation Protocol, the making of centiSperm

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Electroporation, the making of centiSperm

Comic Rendition:  Matteo Farinella

Matteo Farinella of http://matteofarinella.com

A – Mix sperm and DNA in the electroporation vial (cuvette)

For each reaction mix 10 microliters of centipede DNA and 50 microliters of desalinated sperm directly inside the electroporation cuvette

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B – Put cuvette inside of the electroporator

C – Set voltage

I did one reaction at 200 volts which seemed standard in the sperm electroporation. I also ran a second reaction at 1800 volts just to make it rough. Sometimes when you are running a rough DIY lab its better to go for overkill than treading to closely to the protocol. More is sometimes a matter of affect. But more actually often does the trick!

D – Press button twice to fire machine. Wait for beep (about 2 seconds)
E – apply refresh media

For this lab I used 10% vitaminwater® XXX açai-blueberry- pomegranate flavored (Glaceau) put through a 3 micron Gelman Filter

 

 

 

 

 

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The refresh media was prepared before the electroporation reaction.

 

 

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photo credit: David Bowen

 

 

 

 

 

 

 

 

electroporator

     centiSperm as a new media is now available for experimentation.   

 

 

 

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Adam Zaretsky, Ph.D., CEO psyFert a Wet-Lab DIY-BIOart Practitioner mixing Ecology, Biotechnology, Non-human Relations, Body Performance and Gastronomy. Zaretsky stages lively, hands-on bioart production labs based on topics such as: foreign species invasion (pure/impure), radical food science (edible/inedible), jazz bioinformatics (code/flesh), tissue culture (undead/semi-alive), transgenic design issues (traits/desires), interactive ethology (person/machine/non-human) and physiology (performance/stress). His art practice focuses on an array of legal, ethical, social and libidinal implications of biotechnological materials and methods with a focus on transgenic humans.

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