Category: Calls & Opportunities

Electroporation Protocol, the making of centiSperm

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Electroporation, the making of centiSperm

Comic Rendition:  Matteo Farinella

Matteo Farinella of http://matteofarinella.com

A – Mix sperm and DNA in the electroporation vial (cuvette)

For each reaction mix 10 microliters of centipede DNA and 50 microliters of desalinated sperm directly inside the electroporation cuvette

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B – Put cuvette inside of the electroporator

C – Set voltage

I did one reaction at 200 volts which seemed standard in the sperm electroporation. I also ran a second reaction at 1800 volts just to make it rough. Sometimes when you are running a rough DIY lab its better to go for overkill than treading to closely to the protocol. More is sometimes a matter of affect. But more actually often does the trick!

D – Press button twice to fire machine. Wait for beep (about 2 seconds)
E – apply refresh media

For this lab I used 10% vitaminwater® XXX açai-blueberry- pomegranate flavored (Glaceau) put through a 3 micron Gelman Filter

 

 

 

 

 

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The refresh media was prepared before the electroporation reaction.

 

 

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photo credit: David Bowen

 

 

 

 

 

 

 

 

electroporator

     centiSperm as a new media is now available for experimentation.   

 

 

 

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centiSperm DIY Protocol: Centipede DNA Isolation

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Centipede DNA Isolation

A – Kill Centipede
B – Mash with mortar and pestle

This was no easy feat. The night I scanned the centipede the other residents named him Keith! And Keith nearly escaped. I fed and housed him, changed her stream water and caught it bugs. I even found that he preferred raw meat but not fat. With a name and care, Keith felt more like a pet. Also, the skittering, curious and loungy attitude of Keith suggested a fine sentient personality. The antennas poking and wriggling in unison and independently spoke tomes. Even the fear of a venomous organism showing signs of extremely honed escape tactics gave me vast respect to the centipedes of this world.

There is no respectful death possible for an organism to be used for bioart. Still, a good death was considered. There is little written about insect euthanasia. We considered drug overdose but it was unknown if insects responded to diazepam derivatives. I was of the notion that fast, near instantaneous destruction was the best method. Its just a bit of that ultraviolence, smash and grind will do the trick. But, we did try out cryanesthesia (freezing) in the hopes that Keith would be numb. As usual with these death rituals, it is not easy.

Comix Version:  Matteo Farinella of http://www.neurocomic.org/

Comix Version:
Matteo Farinella of http://matteofarinella.com/

Ooops, lame research revealed belatedly:

“The British and Irish Association of Zoos and Aquariums (BIAZA) Terrestrial Invertebrate Working Group (TIWG) reports on a survey conducted by Mark Bushell of BIAZA institutions. He found that refrigeration and freezing were the most common methods “of euthanasia of invertebrates although research has suggested that this is probably one of the least ethical options.” That said, freezing is a worst-case method if chemical or instantaneous physical destruction is not possible.”
- Sarah Pellett; Romain Pizzi; Steven Trim; Mark Bushell; Dave Clarke; Jamie Wood (Jun 2015). BIAZA Recommendations for Ethical Euthanasia of Invertebrates (2.6 ed.). British and Irish Association of Zoos and Aquariums.

Keith began to wake from his semi-frozen slumber as the brass mortar came down on her in the porcelain pestle. Keith was mashed in seconds and the interior looked a lot like blended shrimp meat and the only recognizable remnants were some legs that stuck to the side of the pestle and a long sheath of segmented centipedal chiton all covered in goo.

Keith, mortal, pestle Photo credit: David Bowen

Keith, mortal, pestle
Photo credit: David Bowen

centiMash Photo credit: David Bowen

centiMash
Photo credit: David Bowen

C – Mix mashed centipede with distilled water, salt and a tiny drop of soap
D – let centipede mash sit in hot water bath for 5-10 minutes

I had earlier determined that hypodermic syringes do melt in boiling water. So, I mixed the centipede mash in a tube that had contained a really nice SF styled joint with some remnant cannabis in the bottom.

 

 

Actually, the Cannabis Indica genome was isolated with the centipede genome and they both live on in the centiSperm to some extent.

 

 

 

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I will resist calling this project centi-midnightToker-Sperm for lack of text space on the interwebs. I did make a tube floatation device for the hot water bath. I used plastic netting and corks. There are many corks at the Djerassi Residency, many!
E – Spin to make pellet
F – This time keep the supernatant and throw away the pellet
G – Move liquid to new tube
H – Mix 50/50 with high proof vodka or Everclear
I – Spin at high speed for 10+ minutes
J – Pour off the vodka super natant
K – let the pellet dry aerating until there is no alcohol left
L – Resuspend in 100 microliters of distilled water by pipetting up and down over and over until the DNA pellet is mixed in with the water.

For this step I used my hand held 20-200 microliter pipette but I had not remembered to bring sterile pipette tips. Well I cut some myself from disposable pipettes. I know they are not calibrated but they sure work well and the proportions were right!

DIY Pipette Tips Photo Credit: David Bowen

DIY Pipette Tips
Photo Credit: David Bowen of http://www.dwbowen.com/

Faculty Job Listing: Assistant Professor, American Art, 1945 – Present

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Assistant Professor, Tenure-track, American Art, 1945 – Present
University of Delaware, Newark, Delaware

The Department of Art History at the University of Delaware invites applications for a full-time, tenure-track assistant professor in the history of art from 1945 to the present with specialization in American art. We seek candidates who approach the history of American art within a global context. We are committed to hiring a scholar who shows exceptional promise as an intellectual leader. The successful applicant will join a department that is about to celebrate the 50th anniversary of its establishment and its doctoral program and is recognized as a leader in American art and architectural history.

RESPONSIBILITIES:
In addition to pursuing an ambitious research agenda, the appointee will teach undergraduate and graduate courses from the mid-twentieth century to the present and supervise masters and doctoral students. The Department of Art History offers comprehensive training in the historical and geographical scope of the discipline and recently launched a Curatorial Track Ph.D. program. The ideal candidate will enhance these strengths and engage University-wide initiatives in globalism, diversity, public humanities, and/or digital humanities. The University of Delaware offers many opportunities for interdisciplinary outreach, including partnerships with the departments of Art, Art Conservation, Black American Studies, Women and Gender Studies, and the Winterthur Program in American Material Culture, as well as programs in area studies, design, Museum Studies, and the University Museums. We seek a faculty member who will make the most of these collaborative opportunities.

QUALIFICATIONS:
Ph.D. required.

TO APPLY:
The appointment will begin on September 1, 2015. Ph.D. required. Application deadline: September 12, 2014. Applicants should visit www.udel.edu/udjobs and read “Applicant Instructions” under the “Resources for Applicants” tab before submitting their application. Applicants are asked to create and upload a single document that includes a cover letter and a c.v. Please also arrange for one writing sample and three letters of recommendation to be sent via e-mail or regular mail by September 12, 2014 to Professor Wendy Bellion, Chair, Search Committee, Department of Art History, 318 Old College, 18 East Main Street, Newark, Delaware 19716-2516 (hwbellion@udel.edu). Incomplete dossiers will not be reviewed. Application materials will be shared with the faculty of the Department of Art History.

Founded in 1743, the University of Delaware is one of the nation’s oldest institutions of higher education and combines tradition and innovation. Please visit our website at www.udel.edu.

Application deadline: September 12, 2014
For a complete list of requirements and job duties and to apply online,
please visit the UDJOBS website at a href=”http://www.udel.edu/udjobs” title=”www.udel.edu/udjobs” target=”_blank”>www.udel.edu/udjobs Job ID 102175

Equal Employment Opportunity
The University of Delaware is an Equal Opportunity Employer which encourages applications from minority group members, women, individuals with a disability and veterans. The University’s Notice of Non-Discrimination can be found at www.udel.edu/aboutus/legalnotices.html. Employment offers will be conditioned upon successful completion of a criminal background check. A conviction will not necessarily exclude you from employment.

LMJ25 Call for Papers: The Politics of Sonic Art

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Call for Papers: LMJ25 — The Politics of Sonic Art

For Volume 25 of Leonardo Music Journal we are soliciting articles (papers of up to 3,000 words) and shorter statements (750-1,000 words) that address the role of politics in the creation and dissemination of music and related sonic arts, especially in those genres employing new technological tools.
Possible areas of attention include (but are not limited to):

  • The mechanisms by which sonic art can represent and communicate political content without recourse to language.
    Creative activity under precarious conditions in zones of war, repression and censorship.
  • Historical and political divisions between the categories of technician, composer, and inventor in electronic music studios and live performance.
  • How gendered, racial, cultural, and class privileges operate in technologically driven music.
  • The relationship of phonography and sound walks to environmental activism.
  • The ecological politics of computers and other of short-lifespan materials (recycling vs. landfills).
  • The development of alternative music controllers in response to disabilities.
  • Who benefits and who loses in a file-sharing market?

DEADLINES

15 October 2014: Rough proposals due.

2 January 2015: Submission of finished articles.

Address proposals and inquiries to Nicolas Collins, Editor in Chief, at: ncollins@saic.edu.

Editorial guidelines and information for authors can be found at www.leonardo.info/Authors.

Note: LMJ is a peer-reviewed journal. All manuscripts are reviewed by LMJ editors, editorial board members and/or members of the LMJ community prior to acceptance.